Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK

liangliang Gao; E. M. Babiker; I. C. Nava; Jayaveeramuthu Nirmala; Z. Bedo; L. Lang; S. Chao; S. Gale; Yue Jin; James A Anderson; U. Bansal; R. F. Park; Matthew N Rouse; J. M. Bonman; H. Bariana

doi:10.1111/ppa.12928

Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK

liangliang Gao, E. M. Babiker, I. C. Nava, Jayaveeramuthu Nirmala, Z. Bedo, L. Lang, S. Chao, S. Gale, Yue Jin, James A Anderson, U. Bansal, R. F. Park, Matthew N Rouse, J. M. Bonman, H. Bariana

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt), race TTKSK and related races pose a serious threat to world wheat production. Knowing the effectiveness of wheat stem rust resistance (Sr) genes against Pgt race TTKSK is fundamental in mitigating this threat through resistance breeding. Sr15 was previously identified as being ineffective against Pgt race TTKSK. Here, multirace disease phenotyping data, linkage analyses, allelism testing and haplotype analyses are presented to support the conclusion that Sr15 is effective against Pgt race TTKSK. Resistance to race TTKSK was mapped to Sr15 in a biparental population. Thirty-two accessions with Sr15 displayed seedling resistance phenotypes against race TTKSK. However, these accessions were susceptible as seedlings at high temperatures (22–25 °C), consistent with previous reports that the interaction between avirulent Pgt isolates and Sr15 is temperature-sensitive. Markers STS638, wri4 and KASP_IWB30995 were found to predict the presence of Sr15, suggesting the utility of these assays for marker-assisted selection in breeding programmes. The effectiveness of Sr15 to specific Pgt races and temperatures makes it a less-desirable TTKSK-effective gene. Wheat lines assayed as resistant to race TTKSK at the seedling stage may possess Sr15 and breeders should be aware of the limitations of Sr15 for conferring stem rust resistance.

Original language	English (US)
Pages (from-to)	143-151
Number of pages	9
Journal	Plant Pathology
Volume	68
Issue number	1
DOIs	https://doi.org/10.1111/ppa.12928
State	Published - Jan 2019

Bibliographical note

Funding Information:
The authors would like to acknowledge Sam Stoxen and Samantha Armintrout for technical assistance and Dr Robert Bowden who provided wheat line U5616-20-9-15R. Funding for this research was provided by USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, USAID Feed the Future, the Durable Rust Resistance in Wheat (DRRW) and Delivering Genetic Gain in Wheat (DGGW) projects funded by the Bill and Melinda Gates Foundation and the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. Computing resources from the Minnesota Supercomputing Institute (MSI) at the University of Minnesota are greatly appreciated. R.F.P. gratefully acknowledges support by the Australian-American Fulbright Commission. The authors thank the Australian Grains Research and Development Corporation for funding part of this study. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other product or vendors that also may be suitable.

Publisher Copyright:
Published 2018. This article is a U.S. Government work and is in the public domain in the USA

Keywords

Sr15
Ug99
resistance gene
stem rust
temperature
wheat

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Gao, L., Babiker, E. M., Nava, I. C., Nirmala, J., Bedo, Z., Lang, L., Chao, S., Gale, S., Jin, Y., Anderson, J. A., Bansal, U., Park, R. F., Rouse, M. N., Bonman, J. M., & Bariana, H. (2019). Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK. Plant Pathology, 68(1), 143-151. https://doi.org/10.1111/ppa.12928

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title = "Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK",

abstract = "The wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt), race TTKSK and related races pose a serious threat to world wheat production. Knowing the effectiveness of wheat stem rust resistance (Sr) genes against Pgt race TTKSK is fundamental in mitigating this threat through resistance breeding. Sr15 was previously identified as being ineffective against Pgt race TTKSK. Here, multirace disease phenotyping data, linkage analyses, allelism testing and haplotype analyses are presented to support the conclusion that Sr15 is effective against Pgt race TTKSK. Resistance to race TTKSK was mapped to Sr15 in a biparental population. Thirty-two accessions with Sr15 displayed seedling resistance phenotypes against race TTKSK. However, these accessions were susceptible as seedlings at high temperatures (22–25 °C), consistent with previous reports that the interaction between avirulent Pgt isolates and Sr15 is temperature-sensitive. Markers STS638, wri4 and KASP_IWB30995 were found to predict the presence of Sr15, suggesting the utility of these assays for marker-assisted selection in breeding programmes. The effectiveness of Sr15 to specific Pgt races and temperatures makes it a less-desirable TTKSK-effective gene. Wheat lines assayed as resistant to race TTKSK at the seedling stage may possess Sr15 and breeders should be aware of the limitations of Sr15 for conferring stem rust resistance.",

keywords = "Sr15, Ug99, resistance gene, stem rust, temperature, wheat",

author = "liangliang Gao and Babiker, {E. M.} and Nava, {I. C.} and Jayaveeramuthu Nirmala and Z. Bedo and L. Lang and S. Chao and S. Gale and Yue Jin and Anderson, {James A} and U. Bansal and Park, {R. F.} and Rouse, {Matthew N} and Bonman, {J. M.} and H. Bariana",

note = "Funding Information: The authors would like to acknowledge Sam Stoxen and Samantha Armintrout for technical assistance and Dr Robert Bowden who provided wheat line U5616-20-9-15R. Funding for this research was provided by USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, USAID Feed the Future, the Durable Rust Resistance in Wheat (DRRW) and Delivering Genetic Gain in Wheat (DGGW) projects funded by the Bill and Melinda Gates Foundation and the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. Computing resources from the Minnesota Supercomputing Institute (MSI) at the University of Minnesota are greatly appreciated. R.F.P. gratefully acknowledges support by the Australian-American Fulbright Commission. The authors thank the Australian Grains Research and Development Corporation for funding part of this study. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other product or vendors that also may be suitable. Publisher Copyright: Published 2018. This article is a U.S. Government work and is in the public domain in the USA",

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AU - Nava, I. C.

AU - Nirmala, Jayaveeramuthu

AU - Bedo, Z.

AU - Lang, L.

AU - Chao, S.

AU - Gale, S.

AU - Jin, Yue

AU - Anderson, James A

AU - Bansal, U.

AU - Park, R. F.

AU - Rouse, Matthew N

AU - Bonman, J. M.

AU - Bariana, H.

N1 - Funding Information: The authors would like to acknowledge Sam Stoxen and Samantha Armintrout for technical assistance and Dr Robert Bowden who provided wheat line U5616-20-9-15R. Funding for this research was provided by USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, USAID Feed the Future, the Durable Rust Resistance in Wheat (DRRW) and Delivering Genetic Gain in Wheat (DGGW) projects funded by the Bill and Melinda Gates Foundation and the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. Computing resources from the Minnesota Supercomputing Institute (MSI) at the University of Minnesota are greatly appreciated. R.F.P. gratefully acknowledges support by the Australian-American Fulbright Commission. The authors thank the Australian Grains Research and Development Corporation for funding part of this study. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other product or vendors that also may be suitable. Publisher Copyright: Published 2018. This article is a U.S. Government work and is in the public domain in the USA

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N2 - The wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt), race TTKSK and related races pose a serious threat to world wheat production. Knowing the effectiveness of wheat stem rust resistance (Sr) genes against Pgt race TTKSK is fundamental in mitigating this threat through resistance breeding. Sr15 was previously identified as being ineffective against Pgt race TTKSK. Here, multirace disease phenotyping data, linkage analyses, allelism testing and haplotype analyses are presented to support the conclusion that Sr15 is effective against Pgt race TTKSK. Resistance to race TTKSK was mapped to Sr15 in a biparental population. Thirty-two accessions with Sr15 displayed seedling resistance phenotypes against race TTKSK. However, these accessions were susceptible as seedlings at high temperatures (22–25 °C), consistent with previous reports that the interaction between avirulent Pgt isolates and Sr15 is temperature-sensitive. Markers STS638, wri4 and KASP_IWB30995 were found to predict the presence of Sr15, suggesting the utility of these assays for marker-assisted selection in breeding programmes. The effectiveness of Sr15 to specific Pgt races and temperatures makes it a less-desirable TTKSK-effective gene. Wheat lines assayed as resistant to race TTKSK at the seedling stage may possess Sr15 and breeders should be aware of the limitations of Sr15 for conferring stem rust resistance.

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KW - Sr15

KW - Ug99

KW - resistance gene

KW - stem rust

KW - temperature

KW - wheat

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Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK

Abstract

Bibliographical note

Keywords

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