TY - JOUR
T1 - Temporal patterns of colonization and infection with Mycoplasma hyorhinis in two swine production systems in the USA
AU - Clavijo, Maria Jose
AU - Davies, Peter R
AU - Morrison, Robert
AU - Bruner, Laura
AU - Olson, Steve
AU - Rosey, Everett
AU - Rovira, Albert
N1 - Funding Information:
This work was supported by a grant from Zoetis . Rosey Everett is employed by and receives a salary from Zoetis.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/7
Y1 - 2019/7
N2 - Control of Mycoplasma hyorhinis (M. hyorhinis) associated disease is currently hindered by limited knowledge of the epidemiology and ecology of this organism. A prospective longitudinal investigation was conducted to determine the dynamics of M. hyorhinis colonization in two swine production systems. In each system (A, B), 51 young sows (parities 1, 2) and 56 older sows (>parity 2) were selected at farrowing and tested by qPCR of nasal swabs and for antibodies by serum ELISA. From each sow, a piglet was randomly selected, and nasal and serum samples were collected at birth, weaning, and 10 days post-weaning. Two further samplings were performed in the nursery and finishing stages during the high-risk periods for M. hyorhinis-associated disease, and 12 pigs were euthanized and necropsied at these later sampling events. The prevalence of M. hyorhinis colonization in sows was low (<5%). No associations were found between sow parity or sow serum titer and piglet nasal colonization at either birth or weaning. In contrast to the low prevalence (0.95–2.70%) observed in piglets pre-weaning, most pigs became colonized during the first four weeks after weaning and remained positive throughout the nursery and finishing stages. The detection of M. hyorhinis in oral fluids followed similar patterns as those observed using nasal swabs. ELISA results showed decreased detection of maternal antibodies at around 3 weeks of age and a subsequent increase after natural exposure. The role of M. hyorhinis in polyserositis and arthritis was demonstrated in these two herds. Establishing the temporal dynamics of exposure and infection with M. hyorhinis in pigs will enable more strategic implementation of intervention strategies in affected herds.
AB - Control of Mycoplasma hyorhinis (M. hyorhinis) associated disease is currently hindered by limited knowledge of the epidemiology and ecology of this organism. A prospective longitudinal investigation was conducted to determine the dynamics of M. hyorhinis colonization in two swine production systems. In each system (A, B), 51 young sows (parities 1, 2) and 56 older sows (>parity 2) were selected at farrowing and tested by qPCR of nasal swabs and for antibodies by serum ELISA. From each sow, a piglet was randomly selected, and nasal and serum samples were collected at birth, weaning, and 10 days post-weaning. Two further samplings were performed in the nursery and finishing stages during the high-risk periods for M. hyorhinis-associated disease, and 12 pigs were euthanized and necropsied at these later sampling events. The prevalence of M. hyorhinis colonization in sows was low (<5%). No associations were found between sow parity or sow serum titer and piglet nasal colonization at either birth or weaning. In contrast to the low prevalence (0.95–2.70%) observed in piglets pre-weaning, most pigs became colonized during the first four weeks after weaning and remained positive throughout the nursery and finishing stages. The detection of M. hyorhinis in oral fluids followed similar patterns as those observed using nasal swabs. ELISA results showed decreased detection of maternal antibodies at around 3 weeks of age and a subsequent increase after natural exposure. The role of M. hyorhinis in polyserositis and arthritis was demonstrated in these two herds. Establishing the temporal dynamics of exposure and infection with M. hyorhinis in pigs will enable more strategic implementation of intervention strategies in affected herds.
KW - Swine
KW - arthritis
KW - epidemiology
KW - mycoplasma hyorhinis
KW - polyserositis
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U2 - 10.1016/j.vetmic.2019.05.021
DO - 10.1016/j.vetmic.2019.05.021
M3 - Article
C2 - 31213266
AN - SCOPUS:85066753643
SN - 0378-1135
VL - 234
SP - 110
EP - 118
JO - Veterinary Microbiology
JF - Veterinary Microbiology
ER -