Abstract
Successful advancement in the treatment of diabetes mellitus is not possible without well-established methodology for beta cell mass calculation. Here, we offer the protocol to assess beta cell mass during embryonic development in the mouse. The described protocol has detailed steps on how to process extremely small embryonic pancreatic tissue, cut it on the cryostat, and stain tissue slides for microscopic analysis. The method does not require usage of confocal microscopy and takes advantage of enhanced automated image analysis with proprietary as well as open-source software packages.
| Original language | English (US) |
|---|---|
| Article number | e4641 |
| Journal | Bio-protocol |
| Volume | 13 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 20 2023 |
Bibliographical note
Publisher Copyright:Copyright: © 2023 The Authors; exclusive licensee Bio-protocol LLC.
Keywords
- Beta cell mass
- Embryonic pancreas
- Frozen sections
- Insulin area
- Timed pregnancy
