The crystal structure of the human smacovirus 1 Rep domain

Lidia K. Limón; Ke Shi; Amy Dao; Jacob Rugloski; Kassidy J. Tompkins; Hideki Aihara; Wendy R. Gordon; Robert L. Evans

doi:10.1107/S2053230X23009536

The crystal structure of the human smacovirus 1 Rep domain

Lidia K. Limón, Ke Shi, Amy Dao, Jacob Rugloski, Kassidy J. Tompkins, Hideki Aihara, Wendy R. Gordon, Robert L. Evans

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Abstract

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

Original language	English (US)
Pages (from-to)	295-300
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	79
Issue number	Pt 12
DOIs	https://doi.org/10.1107/S2053230X23009536
State	Published - Dec 5 2023

Bibliographical note

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© 2023 International Union of Crystallography. All rights reserved.

Keywords

bioconjugation
crystal structure
HUH endonucleases
HUH-tags
Rep domains
smacoviruses
ssDNA

PubMed: MeSH publication types

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abstract = "Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 {\AA} resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.",

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AU - Shi, Ke

AU - Dao, Amy

AU - Rugloski, Jacob

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AU - Aihara, Hideki

AU - Gordon, Wendy R.

AU - Evans, Robert L.

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KW - HUH-tags

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KW - smacoviruses

KW - ssDNA

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The crystal structure of the human smacovirus 1 Rep domain

Abstract

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Keywords

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