The detection of alkaline phosphatase using an electrochemical biosensor in a single-step approach

Joanne H. Wang; Kevin Wang; Brandon Bartling; Chung Chiun Liu

doi:10.3390/s91108709

The detection of alkaline phosphatase using an electrochemical biosensor in a single-step approach

Joanne H. Wang, Kevin Wang, Brandon Bartling, Chung Chiun Liu

Anesthesiology

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44 Scopus citations

Abstract

A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0-300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.

Original language	English (US)
Pages (from-to)	8709-8721
Number of pages	13
Journal	Sensors
Volume	9
Issue number	11
DOIs	https://doi.org/10.3390/s91108709
State	Published - Nov 2009

Keywords

Alkaline phosphatase
Biosensor
Electrochemical detection

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abstract = "A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0-300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.",

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AB - A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0-300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.

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The detection of alkaline phosphatase using an electrochemical biosensor in a single-step approach

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