The effect of relaxin on the oxytocin receptor in human uterine smooth muscle cells

Ulrike Friebe-Hoffmann; Dunja M. Baston; Jye Ping Chiao; Liza D. Winebrenner; Jan S. Krüssel; Thomas K. Hoffmann; Jens Hirchenhain; Phillip N. Rauk

doi:10.1016/j.regpep.2006.08.004

The effect of relaxin on the oxytocin receptor in human uterine smooth muscle cells

Ulrike Friebe-Hoffmann, Dunja M. Baston, Jye Ping Chiao, Liza D. Winebrenner, Jan S. Krüssel, Thomas K. Hoffmann, Jens Hirchenhain, Phillip N. Rauk

Obstetrics, Gynecology and Women's Health - Fetal Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Experimental objectives: Activation of the oxytocin receptor (OTR) induces phospholipase C induced PIP₂ turnover in the human uterus. Relaxin (RLX), a polypeptide hormone produced in the corpus luteum of pregnancy as well as in the placenta and decidua inhibits PIP₂ turnover and subsequent signaling in human myometrium. The purpose of this study was to evaluate a possible effect of RLX on OTR regulation in human uterine smooth muscle cells. Primary cultures of myometrium from term pregnant women undergoing elective caesarean section were incubated for different time periods (0-96 h) and with different concentrations of RLX [10 pg/ml-20 μg/ml]. The effects on OTR binding, mRNA and protein expression were evaluated by means of ¹²⁵I-OVT binding assay, RT-PCR and flow cytometry. Results: Prolonged RLX incubation was able to inhibit 30-40% of OTR binding while binding affinity remained unchanged. Oxytocin receptor mRNA and protein expression were down regulated by RLX about 50% and 35% respectively. Conclusion: We report for the first time an effect of RLX on OTR regulation in human uterine myometrial cells. The above results indicate that high local uterine RLX concentrations may be involved in uterine quiescence during human pregnancy by down regulating the OTR.

Original language	English (US)
Pages (from-to)	74-81
Number of pages	8
Journal	Regulatory Peptides
Volume	138
Issue number	2-3
DOIs	https://doi.org/10.1016/j.regpep.2006.08.004
State	Published - Feb 1 2007

Bibliographical note

Funding Information:
U. Friebe‐Hoffmann a was recipient of a scholarship from the German Research Foundation (DFG Fr 1402/1–1) and the Magee‐Women's Research Institute.

Keywords

Insulin-like growth factor family
Peptide regulation
Preterm labor
Signaling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1016/j.regpep.2006.08.004

OpenUrl availability

Full text

Cite this

@article{84474c0d698d46b5a79374cb295a6f01,

title = "The effect of relaxin on the oxytocin receptor in human uterine smooth muscle cells",

abstract = "Experimental objectives: Activation of the oxytocin receptor (OTR) induces phospholipase C induced PIP2 turnover in the human uterus. Relaxin (RLX), a polypeptide hormone produced in the corpus luteum of pregnancy as well as in the placenta and decidua inhibits PIP2 turnover and subsequent signaling in human myometrium. The purpose of this study was to evaluate a possible effect of RLX on OTR regulation in human uterine smooth muscle cells. Primary cultures of myometrium from term pregnant women undergoing elective caesarean section were incubated for different time periods (0-96 h) and with different concentrations of RLX [10 pg/ml-20 μg/ml]. The effects on OTR binding, mRNA and protein expression were evaluated by means of 125I-OVT binding assay, RT-PCR and flow cytometry. Results: Prolonged RLX incubation was able to inhibit 30-40% of OTR binding while binding affinity remained unchanged. Oxytocin receptor mRNA and protein expression were down regulated by RLX about 50% and 35% respectively. Conclusion: We report for the first time an effect of RLX on OTR regulation in human uterine myometrial cells. The above results indicate that high local uterine RLX concentrations may be involved in uterine quiescence during human pregnancy by down regulating the OTR.",

keywords = "Insulin-like growth factor family, Peptide regulation, Preterm labor, Signaling",

author = "Ulrike Friebe-Hoffmann and Baston, {Dunja M.} and Chiao, {Jye Ping} and Winebrenner, {Liza D.} and Kr{\"u}ssel, {Jan S.} and Hoffmann, {Thomas K.} and Jens Hirchenhain and Rauk, {Phillip N.}",

note = "Funding Information: U. Friebe‐Hoffmann a was recipient of a scholarship from the German Research Foundation (DFG Fr 1402/1–1) and the Magee‐Women's Research Institute.",

year = "2007",

month = feb,

day = "1",

doi = "10.1016/j.regpep.2006.08.004",

language = "English (US)",

volume = "138",

pages = "74--81",

journal = "Regulatory Peptides",

issn = "0167-0115",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - The effect of relaxin on the oxytocin receptor in human uterine smooth muscle cells

AU - Friebe-Hoffmann, Ulrike

AU - Baston, Dunja M.

AU - Chiao, Jye Ping

AU - Winebrenner, Liza D.

AU - Krüssel, Jan S.

AU - Hoffmann, Thomas K.

AU - Hirchenhain, Jens

AU - Rauk, Phillip N.

N1 - Funding Information: U. Friebe‐Hoffmann a was recipient of a scholarship from the German Research Foundation (DFG Fr 1402/1–1) and the Magee‐Women's Research Institute.

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Experimental objectives: Activation of the oxytocin receptor (OTR) induces phospholipase C induced PIP2 turnover in the human uterus. Relaxin (RLX), a polypeptide hormone produced in the corpus luteum of pregnancy as well as in the placenta and decidua inhibits PIP2 turnover and subsequent signaling in human myometrium. The purpose of this study was to evaluate a possible effect of RLX on OTR regulation in human uterine smooth muscle cells. Primary cultures of myometrium from term pregnant women undergoing elective caesarean section were incubated for different time periods (0-96 h) and with different concentrations of RLX [10 pg/ml-20 μg/ml]. The effects on OTR binding, mRNA and protein expression were evaluated by means of 125I-OVT binding assay, RT-PCR and flow cytometry. Results: Prolonged RLX incubation was able to inhibit 30-40% of OTR binding while binding affinity remained unchanged. Oxytocin receptor mRNA and protein expression were down regulated by RLX about 50% and 35% respectively. Conclusion: We report for the first time an effect of RLX on OTR regulation in human uterine myometrial cells. The above results indicate that high local uterine RLX concentrations may be involved in uterine quiescence during human pregnancy by down regulating the OTR.

AB - Experimental objectives: Activation of the oxytocin receptor (OTR) induces phospholipase C induced PIP2 turnover in the human uterus. Relaxin (RLX), a polypeptide hormone produced in the corpus luteum of pregnancy as well as in the placenta and decidua inhibits PIP2 turnover and subsequent signaling in human myometrium. The purpose of this study was to evaluate a possible effect of RLX on OTR regulation in human uterine smooth muscle cells. Primary cultures of myometrium from term pregnant women undergoing elective caesarean section were incubated for different time periods (0-96 h) and with different concentrations of RLX [10 pg/ml-20 μg/ml]. The effects on OTR binding, mRNA and protein expression were evaluated by means of 125I-OVT binding assay, RT-PCR and flow cytometry. Results: Prolonged RLX incubation was able to inhibit 30-40% of OTR binding while binding affinity remained unchanged. Oxytocin receptor mRNA and protein expression were down regulated by RLX about 50% and 35% respectively. Conclusion: We report for the first time an effect of RLX on OTR regulation in human uterine myometrial cells. The above results indicate that high local uterine RLX concentrations may be involved in uterine quiescence during human pregnancy by down regulating the OTR.

KW - Insulin-like growth factor family

KW - Peptide regulation

KW - Preterm labor

KW - Signaling

UR - http://www.scopus.com/inward/record.url?scp=33845985642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845985642&partnerID=8YFLogxK

U2 - 10.1016/j.regpep.2006.08.004

DO - 10.1016/j.regpep.2006.08.004

M3 - Article

C2 - 17055075

AN - SCOPUS:33845985642

SN - 0167-0115

VL - 138

SP - 74

EP - 81

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 2-3

ER -

The effect of relaxin on the oxytocin receptor in human uterine smooth muscle cells

Abstract

Bibliographical note

Keywords

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this