TY - JOUR
T1 - The effect of TGF-beta1 on the expression of plasminogen activator inhibitors 1 and matrix metalloproteinases in cultured rat mesangial cells transfected with antisense-TGF-beta1 vector
AU - Lin, W.
AU - Zhang, N.
AU - Zhang, S.
AU - Gu, J.
AU - Guo, M.
PY - 2000/8
Y1 - 2000/8
N2 - OBJECTIVE: To study the plasminogen activator inhibitors 1 (PAI-1) and matrix metalloproteinases (MMPs) expression on the cultured rat mesangial cells (MsC) transfected with antisense-TGF-beta1 vector. METHODS: Lipofectin method was used to transfect antisense-TGF-beta1 vector into MsC, Western blot and Northern blot analysis for detecting TGF-beta1 peptides and antisense TGF-beta1 RNA level. The expression of PAI-1 and MMPs were determined by Northern blot, Western bolt and zymography assay. RESULTS: The MsC clone (MAT1) which blocked the expression of TGF-beta1 peptides was successfully established. The MsC clone showed increased expression of MMP-2 mRNA protein and enzyme activity of MMP-2, 3, 7. However, expression of PAI-1 mRNA of MsC clone was suppressed. CONCLUSION: Blocked expression of TGF-beta1 can upregulate the expression of MMP-2, 3, 7 and downregulate the expression of PAI-1 mRNA in MsC. It is possible that antisense-TGF-beta1 may be used for gene therapy in glomerular diseases.
AB - OBJECTIVE: To study the plasminogen activator inhibitors 1 (PAI-1) and matrix metalloproteinases (MMPs) expression on the cultured rat mesangial cells (MsC) transfected with antisense-TGF-beta1 vector. METHODS: Lipofectin method was used to transfect antisense-TGF-beta1 vector into MsC, Western blot and Northern blot analysis for detecting TGF-beta1 peptides and antisense TGF-beta1 RNA level. The expression of PAI-1 and MMPs were determined by Northern blot, Western bolt and zymography assay. RESULTS: The MsC clone (MAT1) which blocked the expression of TGF-beta1 peptides was successfully established. The MsC clone showed increased expression of MMP-2 mRNA protein and enzyme activity of MMP-2, 3, 7. However, expression of PAI-1 mRNA of MsC clone was suppressed. CONCLUSION: Blocked expression of TGF-beta1 can upregulate the expression of MMP-2, 3, 7 and downregulate the expression of PAI-1 mRNA in MsC. It is possible that antisense-TGF-beta1 may be used for gene therapy in glomerular diseases.
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M3 - Article
C2 - 11866926
AN - SCOPUS:1842865307
SN - 0529-5807
VL - 29
SP - 284
EP - 287
JO - Zhonghua bing li xue za zhi Chinese journal of pathology
JF - Zhonghua bing li xue za zhi Chinese journal of pathology
IS - 4
ER -