Abstract
The effects of sevoflurane, a new volatile anesthetic agent undergoing clinical trial, on the mobilization of intracellular Ca2+ in isolated rat hepatocytes was studied. This agent produced a dose-dependent release of 45Ca2+ from internal, non-mitochondrial stores of permeabilized hepatocytes (saponin treated). However, the administration of sevoflurane to aequorin-loaded intact hepatocytes had little or no effect on intracellular [Ca2+] (i.e., short transient or no increases in luminescence: no toxic effect). These data may indicate that because of the low solubility of sevoflurane, it has a selective effect on endoplasmic reticulum, i.e., mobilizing internal stores of Ca2+ relative to increasing transmembrane fluxes.
Original language | English (US) |
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Pages (from-to) | 81-88 |
Number of pages | 8 |
Journal | Toxicology Letters |
Volume | 66 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1993 |
Bibliographical note
Funding Information:We would like to thank Dr. R.J. Palahniukf or his commentsa, nd R. Nelson, R.A. Olsen and C. Uhl for their technicala ssistanceT.h is work was supportedb y Boehringer Ingerheim,N IH grant GM38033 and NIH Grant CA42286.
Keywords
- Aequorin Ca transient
- Ca release
- Intracellular store
- Isolated hepatocyte
- Rat
- Volatile anesthetic