TY - JOUR
T1 - The human thrombospondin 3 gene
T2 - Analysis of transcription initiation and an alternatively spliced transcript
AU - Adolph, Kenneth W.
AU - Bornstein, Paul
N1 - Funding Information:
This research was supported by a Grant-in-Aid from the University of Minnesota and by National Institutes of Health Grant DE08229.
PY - 1999/7
Y1 - 1999/7
N2 - Thrombospondin 3 (TSP3) is a member of a family of modular, extracellular proteins that have been implicated in a diverse number of important biological processes. To contribute to an understanding of the precise roles of human TSP3, aspects of TSP3 gene transcription have been investigated. The TSP3 gene (THBS3) shares a promoter/intergenic region of 1.4 kb with the divergently transcribed metaxin gene, and the existence of TSP3 transcription initiation sites in the TSP3/metaxin intergenic region was investigated by a PCR procedure. Transcripts were detected which initiate in the intergenic region, up to several hundred bases upstream from the major transcription start site. An alternatively spliced transcript of TSP3 was also detected by the PCR procedure. This includes a new exon, exon A′, which replaces exon A. Exon A′ is located in the TSP3/metaxin intergenic region, 1 kb 5′ of exon A. In addition, transcripts of metaxin were found with extended 5′ ends; these overlap the 5′ end of the TSP3 alternative transcript. The complexities of TSP3 transcription initiation revealed by this study could contribute to the tissue-specific expression and diverse functions of TSP3.
AB - Thrombospondin 3 (TSP3) is a member of a family of modular, extracellular proteins that have been implicated in a diverse number of important biological processes. To contribute to an understanding of the precise roles of human TSP3, aspects of TSP3 gene transcription have been investigated. The TSP3 gene (THBS3) shares a promoter/intergenic region of 1.4 kb with the divergently transcribed metaxin gene, and the existence of TSP3 transcription initiation sites in the TSP3/metaxin intergenic region was investigated by a PCR procedure. Transcripts were detected which initiate in the intergenic region, up to several hundred bases upstream from the major transcription start site. An alternatively spliced transcript of TSP3 was also detected by the PCR procedure. This includes a new exon, exon A′, which replaces exon A. Exon A′ is located in the TSP3/metaxin intergenic region, 1 kb 5′ of exon A. In addition, transcripts of metaxin were found with extended 5′ ends; these overlap the 5′ end of the TSP3 alternative transcript. The complexities of TSP3 transcription initiation revealed by this study could contribute to the tissue-specific expression and diverse functions of TSP3.
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U2 - 10.1006/mcbr.1999.0148
DO - 10.1006/mcbr.1999.0148
M3 - Article
C2 - 10527891
AN - SCOPUS:0033156760
SN - 1522-4724
VL - 2
SP - 47
EP - 52
JO - Molecular Cell Biology Research Communications
JF - Molecular Cell Biology Research Communications
IS - 1
ER -