TY - JOUR
T1 - The influence of macronutrients on splanchnic and hepatic lymphocytes in aging mice
AU - Le Couteur, David G.
AU - Tay, Szun S.
AU - Solon-Biet, Samantha
AU - Bertolino, Patrick
AU - McMahon, Aisling C.
AU - Cogger, Victoria C.
AU - Colakoglu, Feyza
AU - Warren, Alessandra
AU - Holmes, Andrew J.
AU - Pichaud, Nicolas
AU - Horan, Martin
AU - Correa, Carolina
AU - Melvin, Richard G.
AU - Turner, Nigel
AU - O. Ballard, J. William
AU - Ruohonen, Kari
AU - Raubenheimer, David
AU - Simpson, Stephen J.
N1 - Publisher Copyright:
© 2015 The Author.
PY - 2015/12
Y1 - 2015/12
N2 - There is a strong association between aging, diet, and immunity. The effects of macronutrients and energy intake on splanchnic and hepatic lymphocytes were studied in 15 month old mice. The mice were ad-libitum fed 1 of 25 diets varying in the ratios and amounts of protein, carbohydrate, and fat over their lifetime. Lymphocytes in liver, spleen, Peyers patches, mesenteric lymph nodes, and inguinal lymph nodes were evaluated using flow cytometry. Low protein intake reversed aging changes in splenic CD4 and CD8 T cells, CD4:CD8 T cell ratio, memory/effector CD4 T cells and naïve CD4 T cells. A similar influence of total caloric intake in these ad-libitum fed mice was not apparent. Protein intake also influenced hepatic NK cells and B cells, while protein to carbohydrate ratio influenced hepatic NKT cells. Hepatosteatosis was associated with increased energy and fat intake and changes in hepatic Tregs, effector/memory T, and NK cells. Hepatic NK cells were also associated with body fat, glucose tolerance, and leptin levels while hepatic Tregs were associated with hydrogen peroxide production by hepatic mitochondria. Dietary macronutrients, particularly protein, influence splanchnic lymphocytes in old age, with downstream associations with mitochondrial function, liver pathology, and obesity-related phenotype.
AB - There is a strong association between aging, diet, and immunity. The effects of macronutrients and energy intake on splanchnic and hepatic lymphocytes were studied in 15 month old mice. The mice were ad-libitum fed 1 of 25 diets varying in the ratios and amounts of protein, carbohydrate, and fat over their lifetime. Lymphocytes in liver, spleen, Peyers patches, mesenteric lymph nodes, and inguinal lymph nodes were evaluated using flow cytometry. Low protein intake reversed aging changes in splenic CD4 and CD8 T cells, CD4:CD8 T cell ratio, memory/effector CD4 T cells and naïve CD4 T cells. A similar influence of total caloric intake in these ad-libitum fed mice was not apparent. Protein intake also influenced hepatic NK cells and B cells, while protein to carbohydrate ratio influenced hepatic NKT cells. Hepatosteatosis was associated with increased energy and fat intake and changes in hepatic Tregs, effector/memory T, and NK cells. Hepatic NK cells were also associated with body fat, glucose tolerance, and leptin levels while hepatic Tregs were associated with hydrogen peroxide production by hepatic mitochondria. Dietary macronutrients, particularly protein, influence splanchnic lymphocytes in old age, with downstream associations with mitochondrial function, liver pathology, and obesity-related phenotype.
KW - Liver
KW - Lymphocytes
KW - Macronutrients
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U2 - 10.1093/gerona/glu196
DO - 10.1093/gerona/glu196
M3 - Article
C2 - 25335766
AN - SCOPUS:84957793070
SN - 1079-5006
VL - 70
SP - 1499
EP - 1507
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 12
ER -