The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression

John L. Dahl; Kriti Arora; Helena I. Boshoff; Danelle C. Whiteford; Sophia A. Pacheco; Olaus J. Walsh; Dalia Lau-Bonilla; William B. Davis; Anthony G. Garza

doi:10.1128/JB.187.7.2439-2447.2005

The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression

John L. Dahl, Kriti Arora, Helena I. Boshoff, Danelle C. Whiteford, Sophia A. Pacheco, Olaus J. Walsh, Dalia Lau-Bonilla, William B. Davis, Anthony G. Garza

Biology (Duluth)

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of rel_Msm. The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.

Original language	English (US)
Pages (from-to)	2439-2447
Number of pages	9
Journal	Journal of bacteriology
Volume	187
Issue number	7
DOIs	https://doi.org/10.1128/JB.187.7.2439-2447.2005
State	Published - Apr 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1128/JB.187.7.2439-2447.2005

OpenUrl availability

Full text

Cite this

@article{900c448e79404775b09e92995420e1c4,

title = "The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression",

abstract = "The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of relMsm. The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.",

author = "Dahl, {John L.} and Kriti Arora and Boshoff, {Helena I.} and Whiteford, {Danelle C.} and Pacheco, {Sophia A.} and Walsh, {Olaus J.} and Dalia Lau-Bonilla and Davis, {William B.} and Garza, {Anthony G.}",

year = "2005",

month = apr,

doi = "10.1128/JB.187.7.2439-2447.2005",

language = "English (US)",

volume = "187",

pages = "2439--2447",

journal = "Journal of bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "7",

}

TY - JOUR

T1 - The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression

AU - Dahl, John L.

AU - Arora, Kriti

AU - Boshoff, Helena I.

AU - Whiteford, Danelle C.

AU - Pacheco, Sophia A.

AU - Walsh, Olaus J.

AU - Lau-Bonilla, Dalia

AU - Davis, William B.

AU - Garza, Anthony G.

PY - 2005/4

Y1 - 2005/4

N2 - The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of relMsm. The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.

AB - The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of relMsm. The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.

UR - http://www.scopus.com/inward/record.url?scp=15244353229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244353229&partnerID=8YFLogxK

U2 - 10.1128/JB.187.7.2439-2447.2005

DO - 10.1128/JB.187.7.2439-2447.2005

M3 - Article

C2 - 15774887

AN - SCOPUS:15244353229

SN - 0021-9193

VL - 187

SP - 2439

EP - 2447

JO - Journal of bacteriology

JF - Journal of bacteriology

IS - 7

ER -

The relA homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression

Abstract

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this