The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN- independent promoters

D. Foster-Hartnett; R. G. Kranz

doi:10.1128/jb.176.16.5171-5176.1994

The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN- independent promoters

D. Foster-Hartnett, R. G. Kranz

Research output: Contribution to journal › Comment/debate › peer-review

32 Scopus citations

Abstract

The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.

Original language	English (US)
Pages (from-to)	5171-5176
Number of pages	6
Journal	Journal of bacteriology
Volume	176
Issue number	16
DOIs	https://doi.org/10.1128/jb.176.16.5171-5176.1994
State	Published - 1994
Externally published	Yes

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title = "The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN- independent promoters",

abstract = "The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.",

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AB - The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.

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The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN- independent promoters

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