The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans

Karam El-Bayoumy; Chand Sharma; Yves M. Louis; Bandaru Reddy; Stephen S. Hecht

doi:10.1016/0304-3835(83)90100-3

The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans

Karam El-Bayoumy, Chand Sharma, Yves M. Louis, Bandaru Reddy, Stephen S. Hecht

Laboratory Medicine and Pathology

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Abstract

1-Nitropyrene (100 mg/kg body wt.) was administered by gavage to conventional and germfree Fischer 344 rats. 1-Aminopyrene was detected in the feces of conventional rats (5-6% of the dose) but not in the feces of germfree rats. Conjugates of 1-aminopyrene were not detected in the feces of germfree rats and neither 1-aminopyrene nor its conjugates were found in the urine of either conventional or germfree rats. Since reduction of 1-nitropyrene to 1-aminopyrene is an activation process, the results indicate that intestinal microflora are important in the metabolic activation of 1-nitropyrene in vivo in the rat. Human intestinal microflora also reduced 1-nitropyrene to 1-aminopyrene.

Original language	English (US)
Pages (from-to)	311-316
Number of pages	6
Journal	Cancer Letters
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(83)90100-3
State	Published - Jul 1983

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Funding Information:
This study was supported by National Institute of Environmental Health Sciences Grant No. ES-02477 and National Cancer Institute Grant No. CA-35519.

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title = "The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans",

abstract = "1-Nitropyrene (100 mg/kg body wt.) was administered by gavage to conventional and germfree Fischer 344 rats. 1-Aminopyrene was detected in the feces of conventional rats (5-6% of the dose) but not in the feces of germfree rats. Conjugates of 1-aminopyrene were not detected in the feces of germfree rats and neither 1-aminopyrene nor its conjugates were found in the urine of either conventional or germfree rats. Since reduction of 1-nitropyrene to 1-aminopyrene is an activation process, the results indicate that intestinal microflora are important in the metabolic activation of 1-nitropyrene in vivo in the rat. Human intestinal microflora also reduced 1-nitropyrene to 1-aminopyrene.",

author = "Karam El-Bayoumy and Chand Sharma and Louis, {Yves M.} and Bandaru Reddy and Hecht, {Stephen S.}",

note = "Funding Information: This study was supported by National Institute of Environmental Health Sciences Grant No. ES-02477 and National Cancer Institute Grant No. CA-35519.",

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T1 - The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans

AU - El-Bayoumy, Karam

AU - Sharma, Chand

AU - Louis, Yves M.

AU - Reddy, Bandaru

AU - Hecht, Stephen S.

N1 - Funding Information: This study was supported by National Institute of Environmental Health Sciences Grant No. ES-02477 and National Cancer Institute Grant No. CA-35519.

PY - 1983/7

Y1 - 1983/7

N2 - 1-Nitropyrene (100 mg/kg body wt.) was administered by gavage to conventional and germfree Fischer 344 rats. 1-Aminopyrene was detected in the feces of conventional rats (5-6% of the dose) but not in the feces of germfree rats. Conjugates of 1-aminopyrene were not detected in the feces of germfree rats and neither 1-aminopyrene nor its conjugates were found in the urine of either conventional or germfree rats. Since reduction of 1-nitropyrene to 1-aminopyrene is an activation process, the results indicate that intestinal microflora are important in the metabolic activation of 1-nitropyrene in vivo in the rat. Human intestinal microflora also reduced 1-nitropyrene to 1-aminopyrene.

AB - 1-Nitropyrene (100 mg/kg body wt.) was administered by gavage to conventional and germfree Fischer 344 rats. 1-Aminopyrene was detected in the feces of conventional rats (5-6% of the dose) but not in the feces of germfree rats. Conjugates of 1-aminopyrene were not detected in the feces of germfree rats and neither 1-aminopyrene nor its conjugates were found in the urine of either conventional or germfree rats. Since reduction of 1-nitropyrene to 1-aminopyrene is an activation process, the results indicate that intestinal microflora are important in the metabolic activation of 1-nitropyrene in vivo in the rat. Human intestinal microflora also reduced 1-nitropyrene to 1-aminopyrene.

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The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans

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