The Ussing chamber and measurement of drug actions on mucosal ion transport

David R. Brown; Scott M. O'Grady

doi:10.1002/0471141755.ph0712s41

The Ussing chamber and measurement of drug actions on mucosal ion transport

David R. Brown, Scott M. O'Grady

Research output: Contribution to journal › Review article › peer-review

8 Scopus citations

Abstract

Ussing chamber technology can be used to investigate the pharmacodynamic and pharmacokinetic properties of drugs and natural substances on polarized epithelia. With this approach, epithelial sheets or confluent epithelial cell monolayers are mounted between two half-chambers, permitting compound delivery to either the apical or basolateral cell surface. Described in this unit are protocols for determining the effects of test compounds on transepithelial ion transport processes.

Original language	English (US)
Pages (from-to)	7.12.1-7.12.17
Journal	Current Protocols in Pharmacology
Issue number	SUPPL. 41
DOIs	https://doi.org/10.1002/0471141755.ph0712s41
State	Published - Dec 15 2008

Keywords

Airways
Epithelial cell culture
Epithelium
Intestine
Pharmacokinetics
Skin
Transepithelial ion transport

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KW - Airways

KW - Epithelial cell culture

KW - Epithelium

KW - Intestine

KW - Pharmacokinetics

KW - Skin

KW - Transepithelial ion transport

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JO - Current Protocols in Pharmacology

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ER -

The Ussing chamber and measurement of drug actions on mucosal ion transport

Abstract

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