The wag31gene of Mycobacterium tuberculosisis positively regulated by the stringent response

John L. Dahl; Dalia Lau Bonilla

doi:10.1111/j.1574-6968.2011.02278.x

The wag31gene of Mycobacterium tuberculosisis positively regulated by the stringent response

John L. Dahl, Dalia Lau Bonilla

Biology (Duluth)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31_Mtb as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31_Mtb from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31_Mtb expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.

Original language	English (US)
Pages (from-to)	153-159
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	319
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2011.02278.x
State	Published - Jun 2011

Keywords

Mycobacterium smegmatis
Mycobacterium tuberculosis
Stringent response
rel
wag31

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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AB - The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31Mtb as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31Mtb from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31Mtb expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.

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The wag31gene of Mycobacterium tuberculosisis positively regulated by the stringent response

Abstract

Keywords

UN SDGs

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