Abstract
Six strong homologs of the bacterial MutS DNA mismatch repair (MMR) gene have been identified in the yeast Saccharomyces cerevisiae. With the exception of the MSH1 gene, the involvement of each homolog in DNA repair and recombination during meiosis has been determined previously. Five of the homologs have been demonstrated to act in meiotic DNA repair (MSH2, MSH3, MSH6 and MSH4) and/or meiotic recombination (MSH4 and MSH5). Unfortunately the loss of mitochondrial function that results from deletion of MSH1 disrupts meiotic progression, precluding an analysis of MSH1 function in meiotic DNA repair and recombination. However, the recent identification of two separation-of-function alleles of MSH1 that interfere with protein function but still maintain functional mitochondria allow the meiotic activities of MSH1 to be determined. We show that the G776D and F105A alleles of MSH1 exhibit no defects in meiotic recombination, repair base-base mismatches and large loop mismatches efficiently during meiosis, and have high levels of spore viability. These data indicate that the MSH1 protein, unlike other MutS homologs in yeast, plays no role in DNA repair or recombination during meiosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 253-261 |
| Number of pages | 9 |
| Journal | DNA Repair |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 3 2005 |
Bibliographical note
Funding Information:This work was supported by the National Institutes of Health grant GM63626-01 (E.A.S.). E.A.S. is the recipient of a Burroughs-Wellcome Fund Career Award. We thank Scott Keeney (Sloan-Kettering Cancer Center) for the pNKY85 plasmid, Benjamin Kidder (University of Minnesota) for help in strain construction, Peter A. Jauert (University of Minnesota) for assistance with tetrad dissections and Rita Miller (University of Rochester) for use of the Zeiss Axioplan 2 microscope.
Keywords
- MSH1
- Meiosis
- Mismatch repair
- Mitochondria
- MutS
- Recombination