Time Course Metabolite Profiling of Fusarium Head Blight-Infected Hard Red Spring Wheat Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Time of Flight/MS

Wheat is an important food crop, yet its value is reduced by fungal infections (ex. Fusarium graminearum). Metabolite profiling is a useful tool for explaining resistance mechanisms. By analyzing near-isogenic lines (NILs) with contrasting Fhb1 alleles and three wheat varieties, a time course resulting in 61 relevant metabolites was studied. The presence of one metabolite as resistant related constitutive late in the time course was detected. Results confirm the presence of hydroxycinnamic acid amides conjugated with polyamine derivatives (hydroxycinnamic acid amides, HCAAs), which have been shown to induce thickening of cell walls. These compounds are shared by resistant and susceptible genotypes with no difference in intensities but vary in time as early- or late-occurring, suggesting that for the NIL studied here, HCAAs were a normal part of the host reaction. Overall, metabolites synthesized as a result of infection were observed regardless of susceptibility but occurred at different times after infection.