Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells

Joan E. Durbin; Ann Marie Fallon

doi:10.1016/0378-1119(85)90082-4

Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells

Joan E. Durbin, Ann Marie Fallon

Entomology

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.

Original language	English (US)
Pages (from-to)	173-178
Number of pages	6
Journal	Gene
Volume	36
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(85)90082-4
State	Published - 1985

Bibliographical note

Funding Information:
This work was supportedb y a Basil O’Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.

Keywords

Aedes
Drosophila
Recombinant DNA
heat-shock protein promoter
polybrene
transfection

Access

10.1016/0378-1119(85)90082-4

OpenUrl availability

Full text

Cite this

@article{ad64ce217b12412faf1c3d09b12b71ed,

title = "Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells",

abstract = "A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.",

keywords = "Aedes, Drosophila, Recombinant DNA, heat-shock protein promoter, polybrene, transfection",

author = "Durbin, {Joan E.} and Fallon, {Ann Marie}",

note = "Funding Information: This work was supportedb y a Basil O{\textquoteright}Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.",

year = "1985",

doi = "10.1016/0378-1119(85)90082-4",

language = "English (US)",

volume = "36",

pages = "173--178",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells

AU - Durbin, Joan E.

AU - Fallon, Ann Marie

N1 - Funding Information: This work was supportedb y a Basil O’Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.

PY - 1985

Y1 - 1985

N2 - A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.

AB - A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.

KW - Aedes

KW - Drosophila

KW - Recombinant DNA

KW - heat-shock protein promoter

KW - polybrene

KW - transfection

UR - http://www.scopus.com/inward/record.url?scp=0021876240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021876240&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(85)90082-4

DO - 10.1016/0378-1119(85)90082-4

M3 - Article

C2 - 3864716

AN - SCOPUS:0021876240

SN - 0378-1119

VL - 36

SP - 173

EP - 178

JO - Gene

JF - Gene

IS - 1-2

ER -

Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this