Abstract
Intra-thymic injection is a powerful tool for adoptive transfer of cells, cellular tag reagents for tracking recent thymic emigrants (RTEs), or other substances directly into the thymus. The traditional approach developed decades ago requires an invasive surgery to open the thoracic cavity and visualize the thymus. Subsequently, a technique was developed requiring only a small skin incision needed to identify the precise injection site. Nevertheless, both techniques require surgical intervention, and this can lead to elevated animal stress levels and pain which necessitates analgesic medication administration. Here we describe a less invasive technique allowing in situ visualization and transfer of cell suspensions or substances into the thymus via an ultrasound-guided intra-thymic injection approach.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 283-292 |
| Number of pages | 10 |
| DOIs | |
| State | Published - 2023 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2580 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Funding Information:This work was supported by DFG fellowship GE 3062/1-1 to H. Georgiev and by NIH grant R37 AI039560 to K. A. Hogquist.
Publisher Copyright:
© 2023, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
Keywords
- Cell transfer
- Intra-thymic cell injection
- T cell development
- T cell maturation
- Thymus
- Ultrasound guided
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't
- Research Support, N.I.H., Extramural