TY - JOUR
T1 - Unlocking the eukaryotic membrane protein structural proteome
AU - Lee, John Kyongwon
AU - Stroud, Robert Michael
N1 - Funding Information:
This research was supported by National Institute of Health Grant RO1 GM24485, GM73210 and GM74929.
PY - 2010/8
Y1 - 2010/8
N2 - Most of the 231 unique membrane protein structures (as of 3/2010) are of bacterial membrane proteins (MPs) expressed in bacteria, or eukaryotic MPs from natural sources. However eukaryotic membrane proteins, especially those with more than three membrane crossings rarely succumb to any suitable expression in bacterial cells. They typically require expression in eukaryotic cells that can provide appropriate endoplasmic reticulum, chaperones, targeting and post-translational processing. In evidence, only ∼20 eukaryotic MP structures have resulted from heterologous expression. This is required for a general approach to target particular human or pathogen membrane proteins of importance to human health. The first of these appeared in 2005. Our review addresses the special issues that pertain to the expression of eukaryotic and human membrane proteins, and recent advances in the tool kit for crystallization and structure determination.
AB - Most of the 231 unique membrane protein structures (as of 3/2010) are of bacterial membrane proteins (MPs) expressed in bacteria, or eukaryotic MPs from natural sources. However eukaryotic membrane proteins, especially those with more than three membrane crossings rarely succumb to any suitable expression in bacterial cells. They typically require expression in eukaryotic cells that can provide appropriate endoplasmic reticulum, chaperones, targeting and post-translational processing. In evidence, only ∼20 eukaryotic MP structures have resulted from heterologous expression. This is required for a general approach to target particular human or pathogen membrane proteins of importance to human health. The first of these appeared in 2005. Our review addresses the special issues that pertain to the expression of eukaryotic and human membrane proteins, and recent advances in the tool kit for crystallization and structure determination.
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U2 - 10.1016/j.sbi.2010.05.004
DO - 10.1016/j.sbi.2010.05.004
M3 - Review article
C2 - 20739007
AN - SCOPUS:77955981821
SN - 0959-440X
VL - 20
SP - 464
EP - 470
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 4
ER -