Using Microinjection of Mammalian Cultured Cells to Study Cell Division

Charles Day; Alyssa Langfald; Edward H. Hinchcliffe

doi:10.1007/978-1-0716-1904-9_8

Using Microinjection of Mammalian Cultured Cells to Study Cell Division

Charles Day, Alyssa Langfald, Edward H. Hinchcliffe

Hormel Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The introduction of macromolecules directly into individual cells by microinjection is an important technique for manipulating mitotic cells. mRNA, purified proteins, or concentrated antibodies can all be injected directly into a single cell, and their effects monitored by live-cell imaging. The equipment necessary is relatively simple, and the technique can be easily mastered. Here we describe our microinjection setup, how to microinject cultured mammalian cells in mitosis, and how to analyze those cells by same-cell live and fixed imaging.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	105-122
Number of pages	18
DOIs	https://doi.org/10.1007/978-1-0716-1904-9_8
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2415
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Antibody
Cell cycle
Fluorescence
Injector
Micromanipulator
Microneedle
Microscopy
Mitosis
Phosphorylation

PubMed: MeSH publication types

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AU - Hinchcliffe, Edward H.

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KW - Antibody

KW - Cell cycle

KW - Fluorescence

KW - Injector

KW - Micromanipulator

KW - Microneedle

KW - Microscopy

KW - Mitosis

KW - Phosphorylation

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Using Microinjection of Mammalian Cultured Cells to Study Cell Division

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