Using Oct4:MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs)

Lucas V. Greder; Jason Post; James R. Dutton

doi:10.1007/7651_2015_198

Using Oct4:MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs)

Lucas V. Greder, Jason Post, James R. Dutton

Stem Cell Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	97-110
Number of pages	14
DOIs	https://doi.org/10.1007/7651_2015_198
State	Published - Jan 1 2016

Publication series

Name	Methods in Molecular Biology
Volume	1357
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

IPSC
Induced pluripotent stem cell
Lineage tracing
Oct4
Oct4-Cre
Reprogramming

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10.1007/7651_2015_198

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Using Oct4:MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs). / Greder, Lucas V.; Post, Jason; Dutton, James R.
Methods in Molecular Biology. Humana Press Inc., 2016. p. 97-110 (Methods in Molecular Biology; Vol. 1357).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Using Oct4:MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs)

Abstract

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