TY - JOUR
T1 - Whole Exome Sequencing in Patients with Ectopic Posterior Pituitary
AU - Silva, Tatiane S.
AU - Faucz, Fabio R.
AU - Hernández-Ramírez, Laura C.
AU - Pankratz, Nathan
AU - Lane, John
AU - Kay, Denise M.
AU - Lyra, Arthur
AU - Kochi, Cristiane
AU - Stratakis, Constantine A.
AU - Longui, Carlos A.
AU - Mills, James L.
N1 - Publisher Copyright:
© 2022 Published by Oxford University Press on behalf of the Endocrine Society.
PY - 2022/10
Y1 - 2022/10
N2 - Context: Ectopic posterior pituitary (EPP), a condition in which the posterior pituitary gland is displaced due to defective neuronal migration, is frequently associated with hypopituitarism. Genetic variants play a role, but many cases remain unexplained. Objective: A large EPP cohort was studied to explore the importance of genetic variants and how they correlate with clinical findings. Methods: Whole exome sequencing was performed on a discovery sample of 27 cases to identify rare variants. The variants that met the criteria for rarity and biological relevance, or that were previously associated with EPP (ROBO1 and HESX1), were then resequenced in the 27 cases plus a replication sample of 51 cases. Results: We identified 16 different variants in 12 genes in 15 of the 78 cases (19.2%). Complete anterior pituitary deficiency was twice as common in cases with variants of interest compared to cases without variants (9/15 [60%] vs 19/63 [30.1%], respectively; Z test, P=0.06). Breech presentation was more frequent in the variant positive group (5/15 vs 1/63; Z test, P=0.003). Four cases had variants in ROBO1 and 1 in HESX1, genes previously associated with EPP. The ROBO1 p.S18∗ variant has not been reported previously; ROBO1 p.Q1227H has not been associated with EPP previously. Conclusion: EPP cases with variants of interest identified in this study were more likely to present with severe clinical disease. Several variants were identified in genes not previously associated with EPP. Our findings confirm that EPP is a multigenic disorder. Future studies are needed to identify additional genes.
AB - Context: Ectopic posterior pituitary (EPP), a condition in which the posterior pituitary gland is displaced due to defective neuronal migration, is frequently associated with hypopituitarism. Genetic variants play a role, but many cases remain unexplained. Objective: A large EPP cohort was studied to explore the importance of genetic variants and how they correlate with clinical findings. Methods: Whole exome sequencing was performed on a discovery sample of 27 cases to identify rare variants. The variants that met the criteria for rarity and biological relevance, or that were previously associated with EPP (ROBO1 and HESX1), were then resequenced in the 27 cases plus a replication sample of 51 cases. Results: We identified 16 different variants in 12 genes in 15 of the 78 cases (19.2%). Complete anterior pituitary deficiency was twice as common in cases with variants of interest compared to cases without variants (9/15 [60%] vs 19/63 [30.1%], respectively; Z test, P=0.06). Breech presentation was more frequent in the variant positive group (5/15 vs 1/63; Z test, P=0.003). Four cases had variants in ROBO1 and 1 in HESX1, genes previously associated with EPP. The ROBO1 p.S18∗ variant has not been reported previously; ROBO1 p.Q1227H has not been associated with EPP previously. Conclusion: EPP cases with variants of interest identified in this study were more likely to present with severe clinical disease. Several variants were identified in genes not previously associated with EPP. Our findings confirm that EPP is a multigenic disorder. Future studies are needed to identify additional genes.
KW - HESX1
KW - ROBO1
KW - combined pituitary hormone deficiency
KW - ectopic posterior pituitary
KW - exome sequencing
KW - midline defects
KW - pituitary stalk interruption syndrome
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U2 - 10.1210/jendso/bvac116
DO - 10.1210/jendso/bvac116
M3 - Article
C2 - 36042976
AN - SCOPUS:85156154261
SN - 2472-1972
VL - 6
JO - Journal of the Endocrine Society
JF - Journal of the Endocrine Society
IS - 10
M1 - bvac116
ER -