Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Shubhada K. Chothe; Aswathy Sebastian; Asha Thomas; Ruth H. Nissly; David Wolfgang; Maurice Byukusenge; Sunil Kumar Mor; Sagar M. Goyal; Istvan Albert; Deepanker Tewari; Bhushan M. Jayarao; Suresh V. Kuchipudi

doi:10.1016/j.virol.2018.06.015

Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Shubhada K. Chothe, Aswathy Sebastian, Asha Thomas, Ruth H. Nissly, David Wolfgang, Maurice Byukusenge, Sunil Kumar Mor, Sagar M. Goyal, Istvan Albert, Deepanker Tewari, Bhushan M. Jayarao, Suresh V. Kuchipudi

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.

Original language	English (US)
Pages (from-to)	27-36
Number of pages	10
Journal	Virology
Volume	522
DOIs	https://doi.org/10.1016/j.virol.2018.06.015
State	Published - Sep 2018

Bibliographical note

Funding Information:
This work was funded by the Animal Health and Diagnostic Commission, Pennsylvania Department of Agriculture. The authors wish to thank Craig Praul, Kerry Hair, and the staff of the Penn State Genomics Core Facility for library preparation and whole genome sequencing services.

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

BoHV-1
Bovine Herpesvirus-1
Complete genomes
SNP based PCR assay
SNP profiles

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Chothe, S. K., Sebastian, A., Thomas, A., Nissly, R. H., Wolfgang, D., Byukusenge, M., Mor, S. K., Goyal, S. M., Albert, I., Tewari, D., Jayarao, B. M., & Kuchipudi, S. V. (2018). Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1). Virology, 522, 27-36. https://doi.org/10.1016/j.virol.2018.06.015

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abstract = "Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.",

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N2 - Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.

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Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Abstract

Bibliographical note

Keywords

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