TY - JOUR
T1 - Young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in nutrient solution circulating systems
T2 - An alternative, to potted plants and cuttings for mutagenicity tests
AU - Shima, Naoko
AU - Xiao, Ling Zhi
AU - Sakuramoto, Fumihito
AU - Ichikawa, Sadao
N1 - Funding Information:
The present study was supported in part by the Grants-in-Aid for Scientific Research (Nos. 05454607 and 08680565) from the Ministry of Education, Science, Sports, and Culture of Japan to SI.
PY - 1997/12/12
Y1 - 1997/12/12
N2 - The use of young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in a nutrient solution circulating (NSC) growth chamber was tested and developed as an alternative method for using Tradescantia plants in mutagenicity testings. The NSC growth chamber was designed for our requirements, based on trial cultivations of the shoots with roots in its smaller-sized prototype. The nutrient solution used was a 1/2500 Hyponex solution. The characteristics of this clone, i.e., many new shoots constantly emerging from the basal nodes one after another and its short height favorable for early flowering, made it possible to prepare many young inflorescence-bearing shoots with roots at one time. A simplified NSC cultivation system could also be developed at a lower cost, and by using it together with the NSC growth chamber, recycling of untreated materials was established for supplying steadily enough amounts of young inflorescence-bearing shoots with roots for mutagenicity testings. Compared with traditional methods of using potted plants or cuttings, the new method exhibited more stable flower production, better stamen-hair growth and a significantly lower spontaneous (background) mutation frequency, and could produce more inflorescences per space. The use of such young inflorescence-bearing shoots with roots was therefore judged to be satisfactory to serve as a new mutagenicity test system alternating with potted plants and cuttings.
AB - The use of young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in a nutrient solution circulating (NSC) growth chamber was tested and developed as an alternative method for using Tradescantia plants in mutagenicity testings. The NSC growth chamber was designed for our requirements, based on trial cultivations of the shoots with roots in its smaller-sized prototype. The nutrient solution used was a 1/2500 Hyponex solution. The characteristics of this clone, i.e., many new shoots constantly emerging from the basal nodes one after another and its short height favorable for early flowering, made it possible to prepare many young inflorescence-bearing shoots with roots at one time. A simplified NSC cultivation system could also be developed at a lower cost, and by using it together with the NSC growth chamber, recycling of untreated materials was established for supplying steadily enough amounts of young inflorescence-bearing shoots with roots for mutagenicity testings. Compared with traditional methods of using potted plants or cuttings, the new method exhibited more stable flower production, better stamen-hair growth and a significantly lower spontaneous (background) mutation frequency, and could produce more inflorescences per space. The use of such young inflorescence-bearing shoots with roots was therefore judged to be satisfactory to serve as a new mutagenicity test system alternating with potted plants and cuttings.
KW - Mutagenicity tester
KW - Nutrient solution circulating system
KW - Shoots with roots
KW - Tradescantia stamen hairs
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U2 - 10.1016/S1383-5718(97)00169-1
DO - 10.1016/S1383-5718(97)00169-1
M3 - Article
C2 - 9465931
AN - SCOPUS:0031566026
SN - 1383-5718
VL - 395
SP - 199
EP - 208
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 2-3
ER -