Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

Esther Gamero-Sandemetrio; Rocío Gómez-Pastor; Emilia Matallana

doi:10.1007/978-1-4939-7111-4_17

Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

Esther Gamero-Sandemetrio, Rocío Gómez-Pastor, Emilia Matallana

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H₂O₂ allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	189-198
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7111-4_17
State	Published - 2017
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1626
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work has been supported by grants AGL2011-24353 and AGL2014-52985-R from the Spanish Ministry of Economy and Competitiveness (MINECO) to E.M., and it has been performed within the Program VLC/Campus, Microcluster IViSoCa (Innovation for a Sustainable Viticulture and Quality), and Microcluster BBLM (Model Yeasts in Biomedicine & Biotechnology). E.G.-S. was a predoctoral fellow of the JAE program from the CSIC (Spanish National Research Council). R.G.-P. was a postdoctoral researcher at Universitat de València.

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Catalase
Electrophoretic isoforms
SOD
Yeast
Zymogram

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10.1007/978-1-4939-7111-4_17

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Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification. / Gamero-Sandemetrio, Esther; Gómez-Pastor, Rocío; Matallana, Emilia.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 189-198 (Methods in Molecular Biology; Vol. 1626).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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abstract = "We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.",

keywords = "Catalase, Electrophoretic isoforms, SOD, Yeast, Zymogram",

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note = "Funding Information: This work has been supported by grants AGL2011-24353 and AGL2014-52985-R from the Spanish Ministry of Economy and Competitiveness (MINECO) to E.M., and it has been performed within the Program VLC/Campus, Microcluster IViSoCa (Innovation for a Sustainable Viticulture and Quality), and Microcluster BBLM (Model Yeasts in Biomedicine & Biotechnology). E.G.-S. was a predoctoral fellow of the JAE program from the CSIC (Spanish National Research Council). R.G.-P. was a postdoctoral researcher at Universitat de Val{\`e}ncia. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",

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N2 - We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

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KW - Electrophoretic isoforms

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KW - Yeast

KW - Zymogram

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ER -

Zymography methods to simultaneously analyze superoxide dismutase and catalase activities: Novel application for yeast species identification

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