TY - JOUR
T1 - A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation
AU - Ohara, Andre
AU - Santos, Jessika Gonçalves Dos
AU - Angelotti, Joelise Alencar Figueira
AU - Barbosa, Paula De Paula Menezes
AU - Dias, Fernanda Furlan Gonçalves
AU - Bagagli, Marcela Pavan
AU - Sato, Hélia Harumi
AU - Castro, Ruann Janser Soares De
N1 - Publisher Copyright:
© 2018, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.
PY - 2018/12
Y1 - 2018/12
N2 - This study reports the use of statistical mixture design as a tool for the simultaneous production of lipase, CMCase, a-amylase, and Β-glucosidase by Aspergillus niger under solid-state fermentation. Wheat bran, soybean meal, cottonseed meal, and orange peel were used as substrates, either individually or combined in different formulations, to study their synergistic or antagonistic effects on production of the enzymes. The highest lipase (323 U g-1) and CMCase (10 U g-1) activities were detected after 48 h, while the maximum activities of a-amylase (18 U g-1) and Β-glucosidase (15 U g-1) occurred at 72 and 96 h, respectively. Considering the substrate formulation, the ternary mixture of wheat bran (1/3), soybean meal (1/3), and cottonseed meal (1/3) was the most versatile, showing production of CMCase (>5 U g-1) and a-amylase (>8 U g-1) at 24 h, lipase (>320 U g-1) at 72 h, and Β-glucosidase (>10 U g-1) at 48 h.
AB - This study reports the use of statistical mixture design as a tool for the simultaneous production of lipase, CMCase, a-amylase, and Β-glucosidase by Aspergillus niger under solid-state fermentation. Wheat bran, soybean meal, cottonseed meal, and orange peel were used as substrates, either individually or combined in different formulations, to study their synergistic or antagonistic effects on production of the enzymes. The highest lipase (323 U g-1) and CMCase (10 U g-1) activities were detected after 48 h, while the maximum activities of a-amylase (18 U g-1) and Β-glucosidase (15 U g-1) occurred at 72 and 96 h, respectively. Considering the substrate formulation, the ternary mixture of wheat bran (1/3), soybean meal (1/3), and cottonseed meal (1/3) was the most versatile, showing production of CMCase (>5 U g-1) and a-amylase (>8 U g-1) at 24 h, lipase (>320 U g-1) at 72 h, and Β-glucosidase (>10 U g-1) at 48 h.
KW - Agroindustrial wastes
KW - Aspergillus niger
KW - Industrial enzymes
KW - Mixture design
KW - Solid-state fermentation
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U2 - 10.1590/1678-457x.17017
DO - 10.1590/1678-457x.17017
M3 - Article
AN - SCOPUS:85063387057
SN - 0101-2061
VL - 38
SP - 131
EP - 137
JO - Food Science and Technology (Brazil)
JF - Food Science and Technology (Brazil)
ER -